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Photoprotective properties of vitamin D and lumisterol hydroxyderivatives. Cell Biochem. Analytical considerations for the biochemical assessment of vitamin D status. Hoeller, U. Application of dried blood spots to determine vitamin D status in a large nutritional study with unsupervised sampling: The Food4Me project. Higashi, T. Advances in determination of vitamin D related compounds in biological samples using liquid chromatography-mass spectrometry: A review.
B Anal. Life Sci. Zhu, Y. Derivatization methods for LC-MS analysis of endogenous compounds. Bioanalysis 7 , — Indian Council of Medical Research. Final draft nutrient requirements and recommended dietary allowances for Indians. Expert Gr. Indian Council Med. Larkin, E. Agreement of blood spot card measurements of vitamin D levels with serum, whole blood specimen types and a dietary recall instrument. PLoS One 6 Carter, G.
Download references. You can also search for this author in PubMed Google Scholar. Concept Development, Consenting and Health check was performed by A. Data collection, method development and data analysis were carried out by R. All authors contributed to the manuscript writing. Correspondence to Anuradha Khadilkar.
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Reprints and Permissions. Lote-Oke, R. Sci Rep 10, Download citation. Received : 29 July Accepted : 28 October Published : 16 November Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
Advanced search. Skip to main content Thank you for visiting nature. Download PDF. Subjects Biochemistry Endocrinology Molecular biology. This article has been updated. Abstract Vitamin D, a secosteroid, plays an important role in several physiological processes, and its deficiency can lead to numerous pathophysiological conditions in humans.
Introduction Vitamin D is a secosteroid that plays a significant role in bone mineralization and remodelling by regulating the calcium and phosphorus homeostatic balance 1. Blood sampling and spotting Healthy adult volunteers age: 22 to 48 years were requested to provide blood samples for the study, as per a protocol which was approved by the Institutional Ethics Committee- Jehangir Clinical Development Centre Private Limited. Blood spot DBS sample preparation From each of the 45 study participants, intravenous samples were collected approximately 3—4 mL using plain and Heparin tubes.
Matrix matched calibration For serum The charcoal treated serum was used for matrix calibration. Extraction of vitamin D For serum The serum extraction method was a modification of a previously described method 9.
Statistical analysis SPSS software was used for data analysis version 26 0. Figure 1. Full size image. Figure 2. Figure 3. Full size table. Figure 4. Inter-day accuracy and precision for serum and DBS samples.
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